Elucidating the mechanism of cellular uptake
Nanoparticles (NPs) are sub-micrometer sized particles, typically less than 500 nm .
Due to their small size they have the capability to cross the highly electrically resistant blood brain barrier as well as tight junctions .
In the study herein, fluorescently tagged 200nm m NPs functionalised with a penetratin were introduced to monolayer fibroblast cells in the presence and absence of a magnetic field.
However, the method of cell uptake in response to both penetratin and a static field and whether the cell indeed uses a different uptake mechanism for either, are unknown.
Therefore, this paper employs two different blockers of endocytosis, Pitstop 2 and Dyngo 4a, in a bid to determine the cellular uptake route.
imaging and improved treatment of disease, with the ultimate aim of producing a more theranostic approach.
Due to their small sizes, the nanoparticles can cross most of the biological barriers such as the blood vessels and the blood brain barrier, thus providing ubiquitous access to most tissues.